Science
CIS-display technology
Isogenica's CIS-display technology is an in vitro display technology that allows the rapid generation of libraries from which it is possible to select polypeptides with high affinity and fine specificity for most targets. CIS-display is based on the activity of RepA, a CIS-acting DNA-binding protein. "CIS-acting" means that RepA only binds the same molecule of DNA from which it was expressed. Using PCR and in vitro transcription and translation, Isogenica is able to rapidly make vast polypeptide libraries in which each polypeptide is linked to its own encoding DNA sequence. For more details relating to CIS display, click here for a downloadable ppt (1.5Mb)
(Figure 1)
Key features of the technology include:
Library generation is in vitro and is not therefore restricted by bacterial
transformation that limits the size and speed of production of phage display libraries. CIS-display
libraries are highly diverse and can contain between 1012 and 1014 different
gene sequences.
Library generation is extremely fast and flexible. A 1013 library can be generated in just a
few hours, allowing Isogenica to create libraries around virtually any protein scaffold in a short space
of time.
Rounds of selections take place in vitro, meaning that a round of selection can be
completed in one working day.
CIS-display is easy to use. It is based on widely employed molecular biology techniques such as
PCR and in vitro transcription and translation.
CIS display selection cycles
Using cycles of affinity selection Isogenica is able to rapidly identify binding polypeptides that have a high affinity and fine specificity for a molecular target
(Figure 2)
For more information about the use of Cis Display to generate novel peptides, follow the link: Odegrip, et al. (2004) Proc. Nat. Acad. Sci. (USA), vol 101, 2806-2810. For a list of further publications click here. For information on our intellectual property click here.
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