Based around the DNA-binding properties of the RepA protein, CIS display allows the panning of polypeptide libraries via the faithful link between each displayed library member and the dsDNA template encoding it.
CIS display exploits the benefits of cell-free systems in allowing routine access to around 1013 library members, whilst the use of a dsDNA template ensures a robust system suited to simple PCR-based product recovery and library construction.
Fast and robust in vitro antibody display system
Up to 1013 library components expressed in vitro
The Advantages of CIS display
- No transformation, unlike phage display
- DNA-based system, not RNA
- Cis-activity of DNA-binding protein RepA creates stable genotype-phenotype linkage
- >1013 expressed in vitro and panned by affinity selection
- Eluted complexes regenerated by simple PCR
- Optimal ligands after 3-5 successive rounds
- Robust methodology and easy technology transfer
Odegrip et al. 2004, PNAS 101, 2806–2810
Eldridge et al. 2009 PEDS 22, 691–698
Mathonet et al., 2011, Chemistry Today, 29 (2)
Patel et al. 2013, PEDS 26, 307-315